Cancer stem cell isolation and characterization from lung cancer cells

Lung cancer is one of the most common cancers in world and a leading cause death. Cancer stem cells (CSCs) are responsible for tumor initiation. CSCs features include self-renewal capacity, differentiation, high invasion-migration, resistance to chemotherapy. characterized by specific surface markers. CD133 widely used as marker many cancers, including lung cancer. CD133-positive an important because they show characteristics CSCs. less sensitive and/or resistant conventional treatments, remaining viable regenerating tumors after treatment. Treatments that can target will be able destroy more effectively, disappearance tumors. Currently, attempts being made develop therapeutics effectively These studies concern isolation characterization In this study, CD133-negative were isolated from H460 cells, large cell model, MACS method. properties investigated tumorSphere formation assay (2% agar plate), hanging drop qPCR positivity was determined 0.3%. It has been observed CSCs-specific properties. gene expression difference between approximately 25-fold. express pluripotency genes (such OCT-4, SOX-2 KLF-4) compared cells.